Figure 5. Cell sheets detachment after mechanical stimulation (n = 3). (A) Detachment of cell sheets from the PDMS-g-PNIPAAm surface of high grafting yield after incubation at 4°C for 10, 14, 18 and 45 minutes. Blue arrows indicate the direction of the detachment of the cell sheet. (B) F-actin staining (green) of cell sheets before and after detachment. (C) Live/dead staining of cell sheets after detachment. The cell sheets were cultured under loaded condition to confluence. Green dotted lines indicate the dividing line between the cell sheet and the blank area. Scale bars: 1 mm (A, C), 200 μm (B). PDMS-g-PNIPAAm: grafting polymerization of poly(N-isopropyl acrylamide) to poly(dimethylsiloxane).