Figure 7. Effect of mechanical stimulation on the osteogenesis of MC3T3-E1 cells. (A–C) Gene expression of type I collagen (Col I), Runt-related transcription factor 2 (Runx2) and osteopontin (OPN) using β-actin as housekeeping gene and the static group as control. Data are expressed as the mean ± SD (n = 3). *P < 0.05 (Student’s t-test). (D) Western blot analysis of Col I, Runx2 and OPN protein expression.
