Table 2. Antibodies for western blot analyses.
Antibody | Species | Concentration | Catalog number | RRID number | Source |
---|---|---|---|---|---|
Col I | Mouse | 1:1000 | ab6308 | AB_305411 | Abcam, Cambridge, UK |
Runx2 | Rabbit | 1:1000 | ab76956 | AB_156595 | Abcam, Cambridge, UK |
OPN | Rabbit | 1:1000 | ab63856 | AB_1524127 | Abcam, Cambridge, UK |
β-Actin | Mouse | 1:1000 | ab8226 | AB_306371 | Abcam, Cambridge, UK |
Goat anti-rabbit horseradish peroxidase secondary antibody | Goat | 1:10000 | 926-80011 | AB_2721264 | LI-COR, Lincoln, NE, USA |
Goat anti-mouse horseradish peroxidase secondary antibody | Goat | 1:10000 | 926-80010 | AB_2721263 | LI-COR, Lincoln, NE, USA |
Note: Col I: type I collagen; OPN: osteopontin; Runx2: Runt-related transcription factor 2.