Fig. 2. Representative sequence analysis pipeline. The enriched aptamer pool(s) from SELEX were sequenced, and the data set was analyzed to produce a list of aptamer sequences and their relative abundance. This list was used to generate a phylogenetic tree and to search for motifs. Combining information from these analyses, candidate aptamers were chosen for further investigation. The predicted 2D structure and sequence similarity to other aptamers were generated based on the candidate aptamer sequence. From the predicted 2D structure, truncations were designed and 3D structure were predicted. At several points (bar graph icon), binding studies were conducted.