Table 5.
Analytical Practice Characteristics
| No. (%) | |
|---|---|
|
| |
| Laboratory performs enrichment for mutant alleles | 101 |
| Yes | 20 (19.8) |
| No | 81 (80.2) |
| Methoda | 100 |
| Non-NGS | 46 (46.0) |
| NGS | 42 (42.0) |
| Both NGS and non-NGS | 12 (12.0) |
| Technologies | 74 |
| PCR-based method | 33 (44.6) |
| Real-time PCR | 19 (25.7) |
| Digital PCR | 11 (14.9) |
| Allele-specific PCR | 3 (4.1) |
| Sequencing-based methods | 32 (43.2) |
| Amplicon-based targeted NGS | 18 (24.3) |
| Hybrid capture-based NGS | 13 (17.6) |
| Sequencing based, other | 1 (1.4) |
| Both methods | 9 (12.2) |
| Digital PCR and amplicon-based targeted NGS | 4 (5.4) |
| Digital PCR and hybrid-capture-based NGS | 3 (4.1) |
| Allele-specific PCR and amplicon-based targeted NGS | 1 (1.4) |
| Other PCR and sequencing based | 1 (1.4) |
Abbreviations: NGS, next-generation sequencing; PCR, polymerase chain reaction.
a
This factor was determined by selecting a laboratory’s unique method(s) by mailing and then selecting the unique methods for all mailings.