Fig. 5.

Accelerated senescence reduces Aβ levels in 5-month-old 5xFAD mice without altering APP processing or expression. a Schematic diagram depicting the sequential extraction of fractions containing detergent-soluble proteins (containing soluble Aβ) and guanidine hydrochloride (GuHCl)-soluble proteins (containing insoluble Aβ). b MSD Electro-Chemiluminescence Immuno-Assay of human Aβ (hAβ) showing soluble and insoluble hAβ40 and hAβ42 levels for mg of total protein in hippocampal extracts from 5-month-old 5xFAD and G3Terc^−/− 5xFAD mice. *P < 0.05, **P < 0.01, ***P < 0.001 (two-tailed Student’s t-test, n = 4–5 mice/group). c Western blot analysis showing relative protein levels of hAβ, hAPP, CTFα, CTFβ, PS1 and PS2 in hippocampal protein extracts from 5-month-old 5xFAD and G3Terc^−/− 5xFAD mice. Actin was used as loading control, and the levels in the control group were set as 100%. **P < 0.01 (two-tailed Student’s t-test, n = 4–8 mice/group). d Western blot analysis showing relative protein levels of hAβ in cortical protein extracts from 5-month-old 5xFAD and G3Terc^−/− 5xFAD mice. Actin was used as loading control, and the levels in the control group were set as 100%. P = 0.06 (two-tailed Student’s t-test, n = 6–7 mice/group). e RT-qPCR analyses of mouse APP (mAPP) and human APP (hAPP) mRNA levels in total brain extracts from 5-month-old 5xFAD and G3Terc^−/− 5xFAD mice. Non-significant (two-tailed Student’s t-test, n = 3–4 mice/group). All data are presented as mean ± SEM