FIG. 6.

Sequence alignment of the C-terminal domains of a subset of the LAP family. All members of the proposed PepB subfamily and its close relatives are presented, and a diverse group of other LAPs chosen to represent some of the major subgroupings predicted by the phylogenetic tree (Fig. 7) is included for comparison. Positions that are identical for all seven PepBs are indicated by black shading. The conserved residues in the PepB subfamily are indicated in the consensus sequence above the alignment, and the residues that are identical in all members of the LAP family are underlined. The asterisks indicate the positions of K310 and the CSATYRK motif. The arrows indicate the metal binding sites. The reported sequence for A. actinomycetemcomitans PepB shows a G rather than an E in the universally conserved NTDAEGR metal binding site (line 3 of the alignment). We believe that this is likely to be a sequencing error in this incomplete genome sequence and have replaced the G with an E. A.aPepB, A. actinomycetemcomitans PepB (NUMG); P.mPepB, P. multocida PepB (NUMG); H.iPepB, H. influenzae PepB (P45334); S.tiPepB, serovar Typhi PepB (SSC); S.tmPepB, serovar Typhimurium PepB (AF201078 [this work]); E.cPepB, E. coli PepB (P37095); Y.pPepB, Y. pestis PepB (SSC); S.pLAP3, S. putrefaciens LAP3 (NUMG); C.cLAP3, C. crescentus LAP3 (NUMG); L.pLAP, L. pneumophila LAP (NUMG); R.cLAP3, R. capsulatus LAP3 (blast search of WIT at http://www.wit.mcs.anl.gov); E.cPepA, E. coli PepA (P11648); P.aPepA, Pseudomonas aeruginosa PepA (AAD04821); B.tLAP, Bos taurus kidney (P00727); A.pLAP, Aeropyrum pernix LAP (NUMG); SynecLAP, Synechocystis sp. LAP (P73971); B.sLAP, Bacillus subtilis LAP (Z99120); S.aLAP, Staphylococcus aureus LAP (NUMG); R.pPepA, Rickettsia prowazekii PepA (P27888); R.cLAP2, R. capsulatus LAP2 (WIT).