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Purification of serovar Typhimurium PepB
| Step | Total protein (mg) | Total activity (μmol min−1) | Sp act (μmol min−1 mg−1) | % Recovery | Fold purification |
|---|---|---|---|---|---|
| Crude extract | 195 | 12,900 | 66 | 100 | |
| Q Sepharose | 85 | 8,500 | 100 | 66 | 1.5 |
| Heat step | 12 | 2,280 | 190 | 18 | 2.8 |
| Superdex | 8 | 1,520 | 190 | 12 | 2.8 |
