FIG. 2.

Alignment of the pertinent region of amino acid sequence from various methyltransferases. The starting amino acid number is shown at the left. An arrow indicates the G98D mutation observed in the BCG MMAS-3 sequence that appears to correlate with production of methoxymycolic acids by M. tuberculosis. Sequences are MMAS-3 (TubercuList (http://bioweb.pasteur.fr/GenoList/TubercuList/) accession number Rv0643c), CMAS-1 (Rv3392c), CMAS-2 (Rv0503c), MMAS-1 (Rv0645c), MMAS-2 (Rv0644c), and MMAS-4 (Rv0642c), as well as the cyclopropane fatty acid synthase from E. coli (CFAS; SwissProt [http://www.ebi.ac.uk/cgi-bin/swissfetch] accession number P30010).