TABLE 1.

Current common single‐cell transcriptome sequencing protocols.

Platforms	Isolation strategies	Cell scale	UMI	Amplification methods	Region
Smart‐seq2	FACS	Small	NO	PCR	Full‐length
MATQ‐seq	FACS	Small	YES	PCR	Full‐length
Drop‐seq	Microdroplets	Large	YES	PCR	3′ end
inDrop	Microdroplets	Large	YES	IVT	3′ end
STRT‐seq	FACS	Small	YES	PCR	5′ end
10x Genomics	Microdroplets	Large	YES	PCR	3′ end
CEL‐seq2	FACS	Small	YES	IVT	3′ end
Seq‐well	Micro‐fluidic	Large	YES	PCR	3′ end
Fluidigm C1	Micro‐fluidic	Small	NO	PCR	Full‐length
Quartz‐Seq2	FACS	Small	YES	PCR	3′ end