. 2023 Apr 18;67(5):e01386-22. doi: 10.1128/aac.01386-22

TABLE 1.

Enzymatic parameters, LTR DNA binding affinity, infectivity, replicative capacity, and resistance to DTG and BIC for WT, R263K, G118R, and G118R plus R263K integrase proteins and viruses

Genotype	Data from cell-free assays^a				Data from cell-based assays^a
	Strand transfer assays^b			LTR DNA binding assay K_d(nM) (IC95)	Infectivity		Relative replicative capacity (IC95) (%)	FC in resistance (IC95)
	Pseudo-V_max (%) (IC95)	Pseudo-K_m (nM) (IC95)	Enzymatic efficiency (pseudo-V_max/pseudo-K_m)	LTR DNA binding assay K_d(nM) (IC95)	Relative half-infectious titer (IC95)	Relative infectivity (%)^c	Relative replicative capacity (IC95) (%)	DTG	BIC
WT	116 (108–125)	24.8 (21.8–28.11)	4.8	15.2 (13.1–17.2)	1 (9.3–1.1)		100 (83–117)	1 (0.7–1.5)	1 (0.8–1.2)
R263K	42 (39.8–44)^d	23.4 (22.2–24.8)	1.8	30.3 (23–37.6)^d	1.5 (1.3–1.7)^d	67	91 (79–104)	1.9 (1–3.4)	2.4 (2.2–2.7)^d
G118R	17.6 (16.2–19.2)^d^,^e	61.5 (49–77)^d^,^e	0.3	27.7 (19.9–35.5)^d	2.8 (2.6–2.9)^d^,^e	36	34 (29–40)^d^,^e	10.9 (6.3–18.5)^d^,^e	3.9 (2–7.7)^d
G118R plus R263K	34 (29.8–38.8)^d^,^e^,^f	110 (63–206)^d^,^e	0.3	77 (63–91)^d^,^e^,^f	3.3 (3.1–3.4)^d^,^e^,^f	30	10 (3–18)^d^,^e^,^f	13.9 (7.8–25.7)^d^,^e	33.7 (9.7–106)^d^,^e^,^f

IC95, 95% confidence interval.

Enzymatic parameters were derived from binding saturation curve fitting.

Calculated from relative half-infectious titers.

Significantly different from WT (P < 0.05).

Significantly different from R263K (P < 0.05).

Significantly different from G118R (P < 0.05).