FIG. 10.
Effect of toxRVv mutation on OMP expression. (A) OMP profiles of toxRVv mutant CMM981 and isogenic wild-type strain MO6-24/O. V. vulnificus strains were grown in 2.5% NaCl heart infusion at 25°C to stationary phase. OMPs were prepared and analyzed by SDS–12% PAGE. The two major bands appeared to have molecular masses of 38 and 36 kDa. Lanes: A, CMM981; B, MO6-24/O. The values on the left are molecular masses in kilodaltons. (B) Amino-terminal amino acid sequences of the two major OMP bands and their similarities to proteins in the sequence databases. Boldface letters indicate identical sequences. Amino acid residue numbers are shown at the beginning and end of each sequence. ZP2, mouse zona pollucida sperm-binding protein 2 precursor.