Figure 3.

A20 binding and inhibitor of nuclear factor kappa B (NF-κB)-1 (ABIN-1) is processed during autophagy. A: Western blot analysis of ABIN-1, p62, and 1A/1B light chain 3B (LC3B) expression in HeLa cells treated with rapamycin (100 nM) and/or Bafilomycin A1 (BafA1) (4 and 8 h, 200 nM; 24 h, 10 nM) for the indicated time points. Quantification of ABIN-1 (B), p62 (C), and LC3B-II (D). The protein levels were normalized to β-actin. Data represent means ± standard deviation (SD); n = 3 replicates; (*P < 0.05, **P < 0.01, and ***P < 0.001).