Extended Data Figure 4. LAG3 and MHCII protein colocalization on cell surface.

a-b, Untreated HuT 78 cells or HuT 78 cells treated with anti-CD3 (1 ug/ml), anti-CD3 /anti-CD28 (1 ug/ml) or PMA (50 ng/ml) for 48h and LAG3 expression was analyzed by flow cytometry. c, Flow cytometry analyses of HLA-DR expression on unstimulated or stimulated Hut-78 cells. The cells were stained with anti-HLA-DR (clone L243, BioLegend, 1:100). d, Subcellular localization of LAG-3 and HLA-DR on HuT-78 cells. Confocal fluorescence images displaying nuclei (blue), LAG-3 (green) and HLA-DR (red) in HuT-78 cells, either untreated or treated with anti-CD3/anti-CD28. Representative confocal images for the individual channels and merged images are shown in the zoomed panel. One of three representative experiments is shown. Horizontal bars in (b) represent the mean and statistics was determined by one-way ANOVA with P values noted in the figure.