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. 2023 May 3;14:1130442. doi: 10.3389/fimmu.2023.1130442

Figure 1.

Figure 1

Generation of CD19 CAR engineered memory-like NK cells. (A) Schematic diagram of generation of CAR MLNK cells and conventional CAR NK cells. NK cells were pre-activated with IL-12, IL-15 and IL-18 or were control treated (IL-15 only) on day -1, stimulated with engineered K562 feeder cells on day 0, and transduced with retroviral vectors on day 4. NK cells were cultured for a total of 14 days to allow for expansion. (B) Schematic representation of CD19 CAR construct containing the CD19 single chain variable fragment (scFv), CD28 transmembrane domain, CD28 and 4-1BB costimulatory domains and signaling domain CD3zeta. (C) Absolute number of CAR NK cells or CAR MLNK cells shown over time (n=4). (D) Representative FACS histogram plots were shown for CAR expression on CAR NK cells, CAR MLNK cells, NT NK cells and NT MLNK cells on day 3 post transduction. (D) Summarized histogram showing the transduction efficiency of CAR on day 3 and day 10 post transduction (n=4). ns, no significance.