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. 2023 May 3;14:1130442. doi: 10.3389/fimmu.2023.1130442

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Copyright © 2023 He, Mai, Pang, Deng, He, Xue, Xu, Zhou, Liu, Xuan, Li and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phenotypic analysis of CAR engineered memory like NK cells. (A) Representative flow cytometry plots showing the distribution of NK cell subtypes in CAR modified memory-like NK cells based on the relative expression of CD56 and CD16 (n = 4). (B) Evolution of CD56^bright CD16⁺, CD56^bright CD16^-, CD56^dim CD16⁺ and CD56^dim CD16^- NK cell subsets in CAR MLNK cells and CAR NK cells over time (n = 4). (C) Expression levels of KIR2DL1, CD94, NKp30, NKp46, NKG2A, NKG2D, NKG2C, CD25, PD1 and TIM3 on CAR MLNK cells and conventional CAR NK cells (n = 4). *P < 0.05; **P < 0.01; ns, no significance.