Skip to main content
. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Nat Rev Mater. 2022 Dec 19;8(2):123–138. doi: 10.1038/s41578-022-00517-x

Figure 4. Methods to purify functionalized DNA origami nanostructures.

Figure 4.

Purification methods aim to separate functionalized nanostructures from other components in the reaction mixture. In pre-assembly workflows, these methods purify away unfolded scaffolds, excess staples, excess functionalized staples, and misfolded nanostructures. In post-assembly workflows, excess conjugates, and possibly catalysts or side products, are purified away from the functionalized nanostructure. a | Affinity purification methods rely on the affinity between a specific tag on nanostructures and an external material, either column walls or magnets. b | Membrane purification methods utilize membranes that separate reaction mixtures components by a size cut-off. c | Sedimentation purification methods utilize the difference in sedimentation rates between components in the reaction mixture during centrifugation, either in buffer or glycerol solutions. d | Size purification methods rely on different retention times of reaction mixture components in different media, such as agarose gels and SEC beads.