Fig. 2. Engraftment of human hepatocyte affects the phase of the liver circadian clock.

(A) Heatmaps of normalized rhythmic mRNA levels (BICW > 0.3, log₂ amplitude > 0.5) in the liver of LMM (black) and LHM (red) murine and human transcript (red) in the liver of LMM and LHM. Genes presented here belonged to model 14 where both LHM-Mu and LHM-Hu ortholog transcripts share the same phase, which is different from LMM. (B) Radial plot distribution of the peak phase of expression for rhythmic genes in the liver of LMM and murine and human orthologs in the liver of LHM from model 14. (C) Rhythmic expression of circadian clock genes for murine (black, LMM) and murine (blue, LHM-Mu) and human (red, LHM-Hu) RNA orthologs in the humanized liver. Data are expressed as means ± SEM of Log₂ counts per million reads mapped (CPM) (n = 12 mice per group, 2 points per replicate). For statistical details, see table S1.