TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype | Source or reference |
|---|---|---|
| S. meliloti | ||
| Rm8002 | Rm1021 phoA | 9 |
| Rm8384 | Rm8002 fix-384::TnphoA (dctA::phoA) | 9 |
| RmF726 | Rm1021 ΔΩ5079-5149::Tn5-233 | 22 |
| E. coli | ||
| KUR1349 | pyrB usp-4 | 2 |
| KUR1351 | KUR1349 out-2 | 2 |
| S17-1 | pro hsdR recA [RP4-2(Tc::Mu) (Km::Tn7)] | 14 |
| JM109 | lac-proAB | 21 |
| Plasmids | ||
| pTH32 | 2.2-kb EcoRI dctA subclone from pTH24 into pRK7813-1 | 22 |
| pTH2C6 | pTH24 dctA::TnphoA dctB+ dctD+ | 22 |
| pCPP33 | IncP LacZ; Tcr | 7 |
| pSM100 | 1.5-kb BamHI dctA subclone from pTH32 into pCPP33 | This work |