TABLE 1.
Plasmids and bacterial strains
| Plasmid or strain | Relevant properties | Source or reference |
|---|---|---|
| Plasmids | ||
| pAVHoxZ+ | SmaI-SalI fragment (1,049 bases) of hoxGZM containing hoxZ inserted into pBluescript II SK− | This study, subclone of SalI fragment inserted into pSelect (p302L) from reference 40 |
| pAVHoxZ− | pSelect with 231-base KpnI fragment deleted from hoxZ | 40 |
| pDB303 | A. vinelandii Rifr genomic DNA fragment in pUC8; Ampr | D. R. Deana |
| pAVHoxZ H→A series | pAVHoxZ+ with H→A individually in positions 33, 74, 98, 136, 191, 194, 208, and 194 plus 208 | This study |
| pAVHoxZ H→Y series | pAVHoxZ+ with H→Y individually in positions 33, 74, 97, 194, and 208 | This study |
| Strains | ||
| A. vinelandii | ||
| DJ (Wt) | Rifs wild-type, high-transformation strain | D. R. Dean |
| DJ (WtR) | RifrA. vinelandii DJ | This study |
| HoxKG−Rifs | Rifs strain with nonfunctional hoxKG genes derived from A. vinelandii DJ | 40 |
| HoxKG−Rifr | Rifr HoxKG− derived from A. vinelandii HoxKG− Rifs | This study |
| HoxZ− Rifs | Rifs strain with nonfunctional hoxZ gene, derived from A. vinelandii DJ transformed with pAVHoxZ− | This study |
| HoxZ− Rifr | Rifr strain with nonfunctional hoxZ gene derived from A. vinelandii DJ transformed with pAVHoxZ− and pDB303 | 40 |
| HoxZ H→A series | RifrA. vinelandii H→A individually in HoxZ positions 33, 74, 98, 136, 191, 194, and 194 plus 208 | This study |
| HoxZ H→Y series | RifrA. vinelandii H→A individually in HoxZ positions 33, 74, 97, 194, and 208 | This study |
| E. coli DH5α | Amps | Gibco BRL |
a
Virginia Polytechnic Institute and State University.