Fig. 6.

(a) Widths of the first jet at fixed delay time of 10 µs. (b) Velocities of the cell-free, cell-laden jet and the separated single-cell. (c) The diameter of the transferred hydrogel spots on the acceptor slide with the variation of the laser focus depth at constant pulse energy of 2 µJ. The jet velocity and the spot diameter are minimal for the high focus depth of 65 µm, when only the first jet is reaching the acceptor. At 39 µm the transferred single cells separated from the first jet and develop with a significantly higher velocity.