TABLE 3.
Relative specific activities of Acinetobacter esterases against 4-nitrophenyl alkanoates
| No. of carbons in alkyl chain | Relative specific activitya
|
|
|---|---|---|
| SalEb | AreAc | |
| 2 | 450 | 1.9 |
| 4 | 100 | 100 |
| 6 | 0.5 | 87 |
| 8 | <0.1 | 6.5 |
| 10 | <0.1 | 1.4 |
| 12 | <0.1 | 0.9 |
| 14 | <0.1 | 0.2 |
| 16 | <0.1 | <0.1 |
Relative to the activity against 4-nitrophenyl butyrate (C4) (set at 100). All reaction rates were based on the average of three measurements, none of which varied by >5%.
Measured in cell extracts of E. coli BL21(pADPW70). The specific activity against 4-nitrophenyl butyrate was 11.2 U/mg of protein.
Measured in cell extracts of E. coli BL21(pADPW40). The specific activity against 4-nitrophenyl butyrate was 38.0 U/mg of protein.