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. 2023 Apr 10;30(5):594–599. doi: 10.1038/s41594-023-00947-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (a) Genomic integration and composition of the plasmid containing the rRNA mimic used for Noc1-Noc2 RNP expression in yeast, followed by schematic of the two-step purification of the Noc1-Noc2 RNP (b) Schematic of RNA extraction from whole cells (see panel c) and the purified Noc1-Noc2 RNP (see panel d). (c) Methylene blue-stained RNA gel and northern blot of RNA extracted from whole cells containing the Noc1-Noc2 RNP. (d) SYBR Green-stained RNA gel and northern blot of RNA extracted from purified Noc1-Noc2 RNP. (e) Representative SYPRO Ruby stained SDS-PAGE of purified fraction containing the Noc1-Noc2 RNP pre-ribosomal particles. Molecular weight markers are indicated on the left (MW) and bait protein (Noc1; red) and other bands were identified by LC-MS/MS analysis of eluate. RNA, protein gels and northern blot experiments (c-e) were repeated multiple times (n>3) with similar results. (f) Representative motion-corrected cryo-EM micrograph of the Noc1-Noc2 RNP particle sample. (g) Six most-populated 2D class averages (1.3 Å/px), extracted box size 440 pixels, mask diameter 480 Å.