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. 2023 Apr 10;30(5):594–599. doi: 10.1038/s41594-023-00947-3

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — (a) Representative SYPRO Ruby stained SDS-PAGE of purified fraction containing the Noc2-Noc3 RNP (State E) pre-ribosomal particles. Molecular weight markers are indicated on the left (MW) and bait protein (Noc2; red) and other bands were identified by LC-MS/MS analysis of the eluate. Protein gel (and purifications) were repeated multiple times (n>3) with similar results. (b) Representative motion-corrected cryo-EM micrograph of the Noc2-Noc3 RNP particle sample. (c) Six most populated 2D class averages (1.3 Å/px), box size 440 pixels, mask diameter 480 Å. (d) Cryo-EM data acquisition and processing workflow to obtain the final reconstruction of the Noc2-Noc3 RNP at a resolution of 3.69 Å. (e) Solvent-corrected FSC curves for the Noc2-Noc3 RNP, calculated in Relion 3.0, with reported resolutions determined at FSC=0.143 upon post-processing. (f) Local resolution estimation displayed in rainbow color on the cryo-EM map of the Noc2-Noc3 RNP. (g) Final cryo-EM reconstruction of the Noc2-Noc3 RNP with rRNA domains, assembly factors and ribosomal proteins (in gray) identified and colored.