Table 1.
Cryo-EM data collection, refinement and validation statistics
| Noc1–Noc2 RNP (EMD-27919), (PDB 8E5T) | Noc2–Noc3 RNP (EMD-27910) | |
|---|---|---|
| Data collection and processing | ||
| Magnification | ×22,500 | ×22,500 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e–/Å2) | 37.9 | 37.9 |
| Defocus range (μm) | −1.0 to −3.0 | −1.5 to −3.0 |
| Pixel size (Å) | 1.3 | 1.3 |
| Symmetry imposed | C1 | C1 |
| Initial particle images (no.) | 977,232 | 669,811 |
| Final particle images (no.) | 158,915 | 75,358 |
| Map resolution (Å) | 4.0 | 3.69 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | 2.8–11.5 | 2.8–8.5 |
| Refinement | ||
| Initial model used (PDB code) | 6C0F | |
| Model resolution (Å) | 3.7 | |
| FSC threshold | 0.143 | |
| Model resolution range (Å) (FSC 0,0.143,0.5) Masked | 3.6/3.7/7.0 | |
| Map sharpening B factor (Å2) | −44.7 | |
| Model composition | ||
| Non-hydrogen atoms | 55,125 | |
| Protein residues | 5158 | |
| Nucleic acid | 968 | |
| Ligand | Zn-1, Mg-33 | |
| B factors (Å2) | ||
| Protein | 83.71/491.41/148.87 | |
| Ligand | 51.94/257.32/115.29 | |
| R.m.s. deviations | ||
| Bond lengths (Å) | 0.004 (0) | |
| Bond angles (°) | 0.927 (5) | |
| Validation | ||
| MolProbity score | 1.44 | |
| Clashscore | 3.96 | |
| Poor rotamers (%) | 0 | |
| Ramachandran plot | ||
| Favored (%) | 96.21 | |
| Allowed (%) | 3.69 | |
| Disallowed (%) | 0.10 | |