Extended Data Fig. 12. Anti-proliferative effects of TSP-1 inhibition in glioblastoma is limited to activity-dependent mechanisms.
Representative confocal images from HFC glioma monoculture showing no significant change in proliferation (as measured by the number of human nuclear antigen (HNA)-positive cells co-labelled with Ki67 divided by the total number of HNA-positive tumour cells counted across all areas quantified) upon pharmacological TSP-1 inhibition using (32 µM) gabapentin (HFC: n = 7; LFC: n = 9 regions, 2 per group; P = 0.50). Red, HNA (human nuclei); white, Ki67. Scale bar, 30 µm. Data presented as mean ± s.e.m. P values determined by two-tailed Student’s t-test. NS, not significant.