Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 2;30(5):584–593. doi: 10.1038/s41594-022-00905-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Extended Data Fig. 6 — a, A view of the IFT-B1 model docked into its density from the bottom (see E). b, A view of the IFT-B1 model docked into its density from the top (see E). c, Cartoon representation of IFT-B showing the views in A-D. d, A side view of the ‘tail’ of IFT-B1 docked into the masked tail refinement (Extended data 2A) map lowpass filtered to 18 Å. The region containing IFT56 was more flexible in the high-resolution average shown in A/B, but is more clearly resolved here. e, A close up view of IFT56 in the masked tail refinement map, showing that the twist in the TPR helix is visible. f, Density for the central unstructured domain of IFT52 (dark blue) is visible in the central pore of IFT88 (cyan), showing that the Alphafold2 prediction agrees with our experimental data. g, The N-terminal CH domain of IFT37 (light green) docks to the exterior face of the first WD domain of IFT80 (dark green) in IFT-B2. h, A proline residue (magenta) creates a kink in each of the IFT57/38 (dark/light green) helices near the contact to the first IFT88. i, The position of D268 in IFT52 highlighted in red, at the interface between IFT-B1 and IFT-B2. D268 in C. reinhardtii corresponds to the D259H mutation in humans²².