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. 2023 Jan 2;30(5):584–593. doi: 10.1038/s41594-022-00905-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 10 — a, Processing workflow of the classification of the IFT-B average to generate the classes in Fig. 5 that show synchronous IFT-A and IFT-B. Scale bars = 10 nm. b, Surface charge representation of IFT139 shows that the IFT81/74 binding site is strongly negatively charged. c, Surface charge representation of IFT81/74 CC5 shows that it is positively charged, facilitating its interaction with IFT139.