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. 2023 Jan 2;30(5):584–593. doi: 10.1038/s41594-022-00905-5

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — a, Workflow depicting the steps involved in averaging the IFT-B1 and IFT-B2 complexes. Processing started in STOPGAP (areas in dotted black line) before proceeding to Relion. The level of binning at each stage is indicated by the outline of the box (colour code top right). All scale bars=10 nm. b, The solvent masks used to refine IFT-B1 (blue) and IFT-B2 (green) separately from each other. c, The solvent masks used to refine the extremities of the IFT-B1 and IFT-B2 complexes, which are poorly resolved when using the masks in B. d,The solvent mask used to classify and refine dynein from IFT-B2. e, Fourier Shell Coefficient (FSC) curve of the IFT-B1 average, as a measure of map resolution. f, FSC curve of the IFT-B2 average.