. 2023 Jan 2;30(5):584–593. doi: 10.1038/s41594-022-00905-5

Table 1.

Cryo-EM data collection, refinement and validation statistics

	IFT-A average	IFT-B1 average	IFT-B2 average
Data collection and processing
Magnification	33,000×	33,000×	33,000×
Voltage (kV)	300	300	300
Tilt range/increments (°)	±60/3	±60/3	±60/3
Electron exposure (e⁻ Å⁻²)	100	100	100
Defocus range (μm)	−3 to −4.5	−3 to −4.5	−3 to −4.5
Pixel size (Å)	3.03	3.03	3.03
Symmetry imposed	C1	C1	C1
Final particle images (number)	3,897	18,216	18,216
Map resolution/FSC threshold (Å)	20.5/0.143	9.9/0.143	11.4/0.143
Refinement
Map sharpening B factor (Å²)	−2,700	−450	−700
Validation
MolProbity score	2.41	2.18	2.18
Clashscore	23.9	16.7	16.7
Poor rotamers (%)	0.12	0.07	0.07
Ramachandran plot
Favored (%)	90.3	92.7	92.7
Disallowed (%)	0.13	0.1	0.1
FSC (model to map; 0.5 threshold)	21.4	10.2	12.1

The Electron Microscopy Data Bank accession codes for IFT-A, IFT-B1 and IFT-B2 are EMD-15980, EMD-15978 and EMD-15979, respectively. The Protein Data Bank codes are 8BDA, 8BD7 and 8BD7, respectively. FSC, Fourier shell coefficient.