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. 2023 May 1;30(5):692–702. doi: 10.1038/s41594-023-00981-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — a–f Heatmap of TS intensity in (a) N = 139, (b) N = 120, (c) N = 122, (d) N = 340, (e) N = 179 and (f) N = 175 cells (rows) in the presence or absence of indicated factors. Yellow: TS fluorescence intensity; blue: region excluded from analysis. g The fraction of cells that activated during 1 hour of imaging when depleting TBP fully (-TBP, both copies tagged for depletion) or partially (reduced TBP, one copy tagged for depletion) using anchor-away. Data is the fraction based on the number of active and inactive cells + /- propagated statistical errors in these numbers. Significance determined by two-sided Fisher’s exact test; *: p < 0.05; ****: p < 0.00005. p-values: +TBP vs partial TBP 0.0062; +/-TBP 7.7 10⁻⁴². h–j The fraction of (h) cells that activated during 1 hour of imaging, (i) the burst duration and (j) the burst intensity, in -RSC&Reduced TBP were as expected based on their individual depletions. k SWI/SNF and simultaneous TBP and SWI/SNF depletion showed increased GAL10 induction time. Significance in (n) determined by two-sided bootstrap hypothesis testing⁵³; n.s.: not significant; ****: p < 0.00005. p-values: +/- SWI/SNF < 10⁻¹⁵; +TBP vs partial TBP < 10⁻¹⁵; +SWI/SNF&TBP vs -SWI/SNF&partial TBP < 10⁻¹⁵. l–p SWI/SNF and simultaneous TBP and SWI/SNF depletion showed (k) increased induction time of GAL10. (l) The fraction of cells that activated during 1 hour of imaging, (m) the burst duration, (n) the time between bursts, (o) the induction time and (p) the burst intensity for single and double depletion of SWI/SNF and reduced TBP. (h)-(j), (l)-(p) Data for active fraction is the fractional change based on number of active and inactive cells + /- propagated statistical errors in the number of active and inactive cells. Data for other parameters are the fractional change based on mean values + /- standard deviation based on 1000 bootstrap repeats. Grey bar: expected effect based on dynamic epistasis analysis.

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