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(A) Schematic of differentiation protocol including scaffold seeding at Stage 5 Day 1 (S5D1). (B) Fluorescent images throughout stage 5 and 6 of differentiation. (C) Gene expression at Stage 6 Day 10 of planar cultured cells compared to planar cells seeded onto a scaffold at Stage 5 Day 1 (n=6 Planar control, n=12 Planar seeded S5D1).
