Figure 4.

(A) Cell number at various time points after seeding cells S6D1 on the scaffold and continuing cell culture on the scaffold. Scaffolds were analyzed right after seeding, at 24, 48, 96, and 240 hours. Stage media was changed on the scaffold similarly to planar culture. (n=5: 2 hours, n=8: 24 hours, n=8: 48 hours, n=8: 96 hours, n=13: 240 hours). **P<0.01, ***P<0.001, ****P<0.0001. (B) Cell number at the end of differentiation (S6D10) when comparing different seeding starting times. Cells seeded at S5D1 are on the scaffold for about 17 days and 10 days for cells seeded at S6D1. Samples taken the day of transplant were after a brief (2 hour) incubation. (n=5 S5D1 seeded scaffold, n=10 S6D1 seeded scaffold, n=5 Seeded day of transplant). **P<0.01.