Figure 4. Pre-treatment of TAM ex vivo with androgen depletion alone and in combination with aPD-1, increases phagocytosis of PTEN/p53-deficient GEMM tumor cells by MHC-II^hi/PD-1^lo and MHC-II^hi/PD-1^hi TAM subsets, respectively.

(A) Schema illustrating pre-treatment of FACS-sorted TAM subsets from PTEN/p53-deficient prostate tumors with copanlisib (C, 100 nM), PD-1 antibody (P, 10 μg/mL) or their combination under normal and AD condition for 24 hours. After PBS wash, treated TAM were co-cultured with CTV dye stained-AC1/SC1 cells for 2 hours. Bar graphs demonstrate fold change (FC) in phagocytosis of AC1 (B) and SC1 (C) cells by MHC-II^hi/PD-1^hi/lo expressing TAM, relative to untreated group (U). n=2 independent experiments. Significances/p-values were calculated by one-way ANOVA and indicates as follows, **p<0.01 and ***p<0.001; ns = not statistically significant.