TABLE 1.
Analysis of various phenotypes in E. coli and V. cholerae expressing wild-type and/or truncated form of vicH
| Strain (relevant genotype) | Phenotype
|
||
|---|---|---|---|
| Motilitya | β-Glucoside utilizationb | Mucoidyc | |
| E. coli | |||
| FB8 (wild type) | + | W | − |
| BE1410 (hns-1001) | − | R | + |
| BE1410/pDIA562d | + | W | − |
| BE1410/pDIA563 | − | R | + |
| V. cholerae | |||
| Classical O395 (wild type) | + | W | − |
| BV1921 (O395/pDIA563) | − | P | +/− |
a
+, motility revealed by the appearance of a swarming ring on semisolid medium; −, no motility.
b
R, β-glucoside metabolism revealed by the appearance of red colonies on MacConkey-salicin agar plates; W, no metabolism by white colonies; P, intermediate metabolism by pink colonies.
c
+, mucoid phenotype observed on MacConkey-salicin agar plates; −, no mucoid phenotype; +/−, intermediate phenotype.
d
Similar results were obtained with plasmid pDIA566.