Lymph nodes were harvested from SPF (black) and NME (green) offspring at 2, 3, or 6 weeks of age and split in half for staining with two flow panels. (A) Leukocytes were identified by CD45.2 expression and enumerated. Number and frequency of eight immune cell types as indicated: (B) Ɣδ T cells, (C) B cells, (D) neutrophils, (E) monocytes/macrophages, (F) DCs, (G) NK cells, (H) CD4 T cells, and (I) CD8 T cells. (J) Leukocyte composition. 10-15 mice/group/time point. Data were collected from three individual experiments per group per age over two years. Example flow plots are from a 6-week-old NME mouse. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.