Number and frequency of (A) naïve, (B) Tcm, and (C) Tem/Teff CD8 T cells in the lymph nodes harvested from SPF (black) and NME (green) offspring at 2, 3, or 6 weeks of age. (D) Lymph node CD8 T cell composition. Number and frequency of (E) naïve, (F) Tcm, and (G) Tem/Teff CD4 T cells in the lymph node. (H) Lymph node CD4 T cell composition. 10-15 mice/group/time point. Data were collected from three individual experiments per group per age over two years. Example flow plots are from a 6-week-old NME mouse. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.