Number and frequency of (A) Treg, (B) Th1, (C) Th17, (D) Th2, (E) Tfh CD4 T cells in the lymph node harvested from SPF (black) and NME (green) offspring at 2, 3, or 6 weeks of age. (F) Lymph node CD4 T cell lineage composition. (G) Lymph node Th1 and Th17 to Treg, Th2, and Tfh ratio. 10-15 mice/group/time point. Data were collected from three individual experiments per group per age over two years. Example flow plots are from a 6-week-old NME mouse. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001.