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. Author manuscript; available in PMC: 2023 Nov 15.
Published in final edited form as: Cancer Res. 2023 May 15;83(10):1684–1698. doi: 10.1158/0008-5472.CAN-22-2433

Figure 3. LSD1-mediated FOXA1 chromatin binding promotes BRD4 recruitment.

Figure 3.

(A) The genome view of indicated ChIP-seq peaks within an identified super-enhancer (SE) upstream of MYC locus (hg19), which contains two genomic regions, SE1 and SE2, based on strong enhancer markers. (B) Top-ranked motifs from published BRD4 ChIP-seq in 22RV1 cells (GSE94013). (C) Stable 22RV1 cells expressing V5-tagged WT FOXA1 or K270R mutant (grown in hormone-depleted medium) were pretreated with doxycycline to induce FOXA1 expression, and then treated with DMSO or ORY-1001 (5μM) for 1 day. (D) Immunoprecipitation (IP) of LSD1 or BRD4 in 22RV1 cells (grown in hormone-depleted medium) followed by immunoblotting of FOXA1, BRD4, and LSD1. (E) IP of BRD4 followed by immunoblotting of FOXA1 in 35CR and 96CR xenograft tissue. (F) Heatmap view of ChIP-seq intensity of LSD1, BRD4, FOXA1, H3K27ac, and H3K4me2 in LNCaP cells (grown in medium with charcoal-striped serum, CSS).