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. 2023 May 17;10:287. doi: 10.1038/s41597-023-02214-y

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — (a) Example image of a mouse on a running disc, under the two-photon microscope objective. (b) Example image of a mouse pupil. (c) (left) Example of a pupil in which 8 poles have been labelled using DeepLabCut to allow pupil position (average position of the 8 poles) and diameter (average length of the 4 diameters formed by the poles) to be estimated. (right) Inferred pupil ellipse.