TABLE 1.
Bacterial strains and plasmids used for complementation analyses
| Strain or plasmid | Description | Source, parent, and/or reference |
|---|---|---|
| Strains | ||
| V. cholerae | ||
| O395N1 | Wild type | |
| VcΔX | ΔmotX | O395N1, this study |
| VcΔY | ΔmotY | O395N1 (20) |
| VcΔAB | ΔpomAB | O395N1, this study |
| VcΔG | ΔfliG | O395N1 (20) |
| VcΔXYAB | ΔmotX ΔmotY ΔpomAB | O395N1, this study |
| VcΔXYABG | ΔmotX ΔmotY ΔpomAB ΔfliG | O395N1, this study |
| HM-1 | Spontaneously hypermotile | VcΔXYABG, pMotAB, pBAD-VcG |
| HM-2 | Spontaneously hypermotile | HM-1 |
| HM-3 | Spontaneously hypermotile | HM-2 |
| E. coli EcΔG | (DFB225) ΔfliG | D. Blair (22) |
| Plasmids | ||
| pACYC184 | Camr, cloning vector | |
| pMotAB | (pJZ19), E. coli fliG in pACYC184 | D. Blair (6) |
| pBAD-24 | Ampr, cloning vector | |
| pBAD-VcG | V. cholerae fliG in pBAD-24 | This study |
| pBAD-EcG | E. coli fliG in pBAD-24 | This study |
| pBAD-FP1 | V. cholerae-E. coli chimeric fliG in pBAD-24 | This study |
| pBAD-FP2 | E. coli-V. cholerae chimeric fliG in pBAD-24 | This study |