TABLE 2.
Strain | Motility
|
|||||
---|---|---|---|---|---|---|
CCCP
|
Phenamil, pH 6.5
|
|||||
pH 6.5
|
pH 8.5
|
|||||
0 | 30 μM | 0 | 30 μM | 0 | 100 μM | |
EcΔG, pBAD-EcG | +++ | − | +++ | − | +++ | +++ |
VcΔG, pBAD-VcG | ++++ | − | ++++ | +++ | ++++ | − |
HM-1 | +++ | − | +++ | − | +++ | +++ |
HM-2 | +++ | − | +++ | − | +++ | +++ |
Motilities of the V. cholerae (VcΔG, pBAD-VcG) and E. coli (EcΔG, pBAD-EcG) control strains as well as two hypermotile derivatives of the V. cholerae hybrid motor strains (HM-1 and HM-2) were assayed under the microscope. Motility was scored as described in Materials and Methods.