Figure S3.

The expression levels of recombinant proteins expressed in the KO cells are similar to those in the parent cells. (A) Western blot analysis of the endogenous CERT and ectopic CERT-mVenus expressed in various cells treated with or without the sphingolipid synthesis inhibitor myriocin. Cells stably expressing CERT-mVenus were cultured with or without 2.5 μM myriocin for 24 h. Cell lysates were immunoblotted with anti-CERT or anti-GAPDH antibodies. Statistical significance was determined by two-sided Dunnett test. *, P < 0.05, **, P < 0.01. (B) Western blot analysis of FLAG-PI4KB stably expressed in various cells. Cell lysates were immunoblotted with anti-FLAG or anti-GAPDH antibodies. Statistical significance was determined by two-sided Dunnett test. n.s., not significant. Source data are available for this figure: SourceData FS3.