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. 2023 May 17;14(5):328. doi: 10.1038/s41419-023-05845-6

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Association between BRCA1 and ABRAXAS1 was examined in LCLs ABR-wt, ABR-1106 and ABR-577 with wild-type ABRAXAS1 (white columns), with ABRAXAS1 c.1106dup (light grey column) and ABRAXAS1 c.577C>T (dark grey column), respectively, via PLA. LCLs were either exposed to IR and re-cultivated for 4 h or left untreated. PLA-foci were scored by automated quantification of 100 nuclei for each time-point in three independent experiments each and normalized to the mean PLA-foci values of the wild-type control ABR-wt 4 h post IR (absolute values corresponding to 100%: PLA, 7.3). Columns show mean values; n = 300; bars, SEM; **P < 0.01, ***P < 0.001; ****P < 0.0001. Statistically significant differences were calculated via Kruskal–Wallis test followed by two-tailed Mann–Whitney U test comparing mean values for different LCLs. a Graphic presentation of ABRAXAS1/BRCA1 PLA-foci per nucleus (%). b Representative images of PLA-foci (red) using primary antibodies anti-BRCA1 (mouse) and anti-ABRAXAS1 (rabbit) in DAPI-stained nuclei (blue). Negative controls without the primary (1st) and secondary (2nd) antibody (Ab), respectively, were performed to validate the specificity of the stainings.