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. 2023 May 17;14:2824. doi: 10.1038/s41467-023-38527-0

Fig. 2. Germ cell proliferative activity differs between wild isolates JU1200 and JU751.

Fig. 2

a Number of PZ nuclei at four developmental stages: mid-L4 larval (mL4), young adult (YA) with 1-10 eggs in utero, adult at 24 hours post-mid L4 (mL4 + 24 h), and adult at 24 hours post-young adult (YA + 24 h). Nuclei were counted in z-stacks of extruded DAPI-stained gonads. Lowercase letters indicate significant Tukey-adjusted pairwise contrasts (p < 0.05), so that groups sharing the same letters are not significantly different. Cross bars and error bars represent estimated marginal means ± standard errors derived from a generalized linear model. n-values for each strain and stage are indicated below the x-axis (JU1200=red, JU751=blue) b Number of EdU+ (positive) nuclei in the PZ after a 15-minute pulse of EdU at the mid-L4 stage (***p = 0.00005, Tukey-adjusted pairwise contrast). n-values are indicated below the x-axis. Cross bars and error bars represent estimated marginal means ± standard errors derived from a generalized linear model. c Representative images of the PZ in whole-mount DAPI-stained worms at mL4 + 24 h. Scale bars: 20μm. d Representative images of EdU staining at the mid-L4 stage. Dotted lines mark the proximal boundary of the PZ. Scale bars: 20μm. Data are shown from a single (out of two) experimental blocks. For data and statistical results, see Supplementary Notes 3 and 4 and the Source Data file.