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. 2023 Apr 20;26(5):106705. doi: 10.1016/j.isci.2023.106705

Figure 2.

Figure 2

Established HM20-T resin embedding method

(A) Principle of redox reactions system. The main principle of this reaction was the reaction of DMT with BPO, which promoted the release of free radicals from BPO and then initiated the polymerization of monomers and crosslinkers.

(B) Polymerization state of the resin embedding sample at different concentrations of DMT and BPO.

(C) Schematic diagram illustrating the procedure for whole-brain resin embedding. We added 0.7% DMT in polymer solution and embedded samples at −20°C.