Figure 1.

Cx43 hemichannels are lateralized in cardiomyocytes of S3A mice. (A) Representative confocal immunofluorescence images of cardiac intercalated discs and lateral regions of cardiomyocytes stained with Cx43 (green) and N-cadherin (red). Yellow and white arrows indicate the intercalated disc and lateral side, respectively. Right: Quantification of Cx43/N-cadherin colocalization in confocal immunofluorescence images. All data points were normalized to the WT group mean. Between three and five images containing 15–20 IDs were analyzed per heart. Each dot represents the mean value for each biological replicate. Comparisons between groups were made using Student’s t test, P = 0.0001 vs. WT. The number in parentheses indicates the n total mice value. (B) Western blot analysis (left) and quantification (right) of Cx43 from biotin-perfused hearts (biotinylation). The bottom row represents Cx43-immunoblotted samples from heart lysates prior to pulldown (total Cx43). Biotinylated Cx43 levels were expressed as fold change relative to total Cx43 protein levels per sample. The number in parentheses indicates the n total mice value. Comparisons between groups were made using Student’s t test, P = 0.0001 vs. WT. Source data are available for this figure: SourceData F1.