Figure S1.

AA diet mice are heathy and display no evidence of intestinal damage or inflammation. (A) Percent of original body weight (top) or fecal lipocalin-2 levels measured by ELISA (bottom) from SPF mice at indicated weeks after weaning onto AA or standard chow diet. Mean ± SEM representative of two independent experiments using 19–22 mice per group. Comparisons between diets within timepoints are not significant as calculated by unpaired t test. (B) Serum nutritional biomarkers measured in 8-wk-old SPF mice fed AA or chow diet since weaning. ALP—alkaline phosphatase, AST—aspartate aminotransferase, BUN—blood urea nitrogen, HDL—high density lipoprotein, LDL—low density lipoprotein, LDH—lactose dehydrogenase. Two independent experiments, each point represents pooled serum from four to six mice. (C–F) SPF or GF mice were fed AA or standard chow diet from weaning until analysis at 8 wk old. (C) Small intestine length. (D) Representative H&E histology images with 200 μm scale bar. (E) H&E pathology scores based on one image per tissue per mouse, where 40 is the maximum score (top left), or tissue morphology measures, where each dot represents the average of four measurements per tissue per mouse. (F) Flow cytometry analysis of myeloid cells in the small intestine IE and LP. (C–F) Mean ± SD representative of two independent experiments with six to nine mice per group. Unpaired t tests with Holm-Šidák multiple comparison test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.