Skip to main content
. 2023 Apr 23;26(5):106723. doi: 10.1016/j.isci.2023.106723

Figure 1.

Figure 1

DAPK1 basal synaptic enrichment requires F-actin, and stabilization of F-actin during cLTP blocks DAPK1 synaptic removal

Scale bars, 5 μm.

(A) Representative images of mCh-DAPK1, mTq-PSD95 intrabody, and mCitrine cell fill. DAPK1 synaptic enrichment values were measured over time following treatment with Latrunculin B (2.5 μM) and washout after aCSF.

(B) Basal synaptic enrichment values of DAPK1, PSD95, and cell fill were compared (n = 24 neurons; ∗∗∗p < 0.001; RM one-way ANOVA with Tukey’s multiple comparisons test). Individual data points are shown; the bar graphs represent mean ± SEM.

(C) Time course of DAPK1, PSD95, and cell fill synaptic enrichment values after treatment with Latrunculin B for 5 min followed by a washout with aCSF (n = 8 neurons). Data are represented as mean ± SEM.

(D and E) (D) cLTP stimulus (100 μM glutamate/10 μM glycine, 1 min) promoted the reduction of DAPK1 synaptic enrichment (n = 13 neurons; ∗∗p < 0.01; paired two-tailed t-test), but (E) stabilization of F-actin with jasplakinolide (1 μM, 10 min) instead blocked DAPK1 synaptic dispersal after cLTP (n = 14 neurons; ∗p < 0.05; paired two-tailed t-test).