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. 2023 Apr 23;26(5):106723. doi: 10.1016/j.isci.2023.106723

Figure 2.

Figure 2

DAPK1 localization to synapses via F-actin is required for initiation of cLTD-specific DAPK1 synaptic pool

Scale bars, 5 μm.

(A) cLTD stimulation (30 μM NMDA/10 μM CNQX/10 μM glycine, 1 min) did not affect DAPK1 synaptic enrichment (n = 9 neurons; paired two-tailed t-test).

(B) cLTD stimulation 1 min prior to application of Latrunculin B impaired the Latrunculin-induced removal of DAPK1 from synapses (n = 15, 16 neurons; ∗∗∗p < 0.001; two-way RM ANOVA). Data are represented as mean ± SEM.

(C) cLTD stimulation following Latrunculin-induced removal of DAPK1 did not result in synaptic re-accumulation of DAPK1 (n = 14 neurons; paired two-tailed t-test).